Illustration of a mass spectrometer setup with a chromatography machine, bottles with buffers, and an antibody diagram, showing data analysis on a screen with a graph of intensity versus mass-to-charge ratio.

Intact Mass Spectrometry

High-resolution analysis of intact proteins to determine molecular mass and evaluate structural integrity.

Workflow:
→ Direct infusion or LC-MS
→ Deconvolution
→ Mass confirmation

Applications:
• Mass confirmation of expressed proteins
• Identification of clipping, aggregation, or degradation
• Detection of high-abundance PTMs
• Batch-to-batch comparison for biologics manufacturing

Illustration of a protein identification process using mass spectrometry. Shows a protein structure, enzyme digestion, peptide separation via chromatography, mass spectrometer analysis, and resulting chromatograms.

Peptide Mapping

Workflow:
→ Proteolytic digestion
→ LC-MS/MS analysis
→ Data interpretation

High-resolution peptide mapping enables confirmation of protein sequence and localization of post-translational modifications (PTMs), forming the backbone of Multi-Attribute Monitoring (MAM) workflows.

Applications:
• Sequence confirmation of recombinant proteins
• Detection and quantitation of PTMs (e.g., oxidation, deamidation, etc.)
• Support for MAM-based QC strategies
• Monitoring and validation of CQAs
• Batch comparability and lot release testing

proteomics experiment depiction including generic proteins entering a mass spec and a plot showing data

Proteomics

Top-down and bottom-up proteomics to characterize proteins in complex samples or purified forms.

Workflow:
→ Sample prep
→ LC-MS/MS (intact or digested)
→ Database search and analysis

Applications:
• Protein identification and quantitation
• Drug target discovery and validation
• PTM analysis
• Functional and interaction profiling
• Biomanufacturing QC

Diagram showing three glycan structures attached to a peptide, depicting variations in glycosylation patterns.

Glyco-Profiling

Mass spectrometry-based identification and characterization of glycan structures on proteins.

Workflow:
→ Enzymatic or chemical release
→ MS/MS analysis
→ Glycan structure annotation

Applications:
• Glycosylation site identification
• Glycan structure and branching analysis
• Quality control in biologics
• Mechanistic studies of disease-related glycosylation

Illustration of antibody structure with hydrogen and deuterium labels during HDX-MS

HDX-MS

Hydrogen Deuterium Exchange Mass Spectrometry reveals protein dynamics, conformational stability, and binding interfaces through deuterium uptake profiling.

Workflow:
→ Deuterium labeling
→ Quenching and digestion
→ LC-MS/MS
→ Uptake comparison

Applications:
• Epitope and paratope mapping
• Protein-ligand and protein-protein interaction studies
• Structural comparability and biosimilarity
• Biologics formulation optimization

Illustration of liquid chromatography LC and Mass spectrometry MS laboratory equipment featuring machinery with digital displays and solvent bottles on top.

Polyclonal Epitope Mapping

A proprietary HDX-MS-based workflow for mapping dominant epitopes within polyclonal serum in complex biological matrices.

Workflow:
→ Serum binding
→ HDX-MS analysis
→ Epitope landscape mapping

Applications:
• Vaccine response profiling
• Polyclonal antibody characterization
• Immunogenicity and serological surveillance.

Diagram illustrating a biosensor with molecules binding to antibodies on a surface, accompanied by graphs showing response over time.

BLI

BioLayer Interferometry is a label-free optical technique that quantifies real-time biomolecular interactions, including association and dissociation kinetics.

Workflow:
→ Ligand immobilization
→ Analyte binding
→ Real-time optical readout

Applications:
• Kinetic (on/off-rate) analysis
• Affinity comparison across variants
• Screening for binding strength and specificity

Let’s Work Together